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13683 1 ap rabbit polyclonal  (Proteintech)


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    Structured Review

    Proteintech 13683 1 ap rabbit polyclonal
    13683 1 Ap Rabbit Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+chmp4b/CHMP4B+Polyclonal+antibody/pmc12542950__sciadv%2Eadv6805_sm-97-36-34
    Average 94 stars, based on 91 article reviews
    13683 1 ap rabbit polyclonal - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: HIV-1 budding requires cortical actin disassembly by the oxidoreductase MICAL1
    Article Snippet: The following antibodies were used for western blot experiments: rabbit anti-MICAL1 (1:500, Proteintech Europe 14818-1-AP), rabbit anti-Rab35 (1:500, Proteintech Europe 11329-2-AP), mouse anti-GAPDH (1:8000 Proteintech Europe 60004-1-Ig), rabbit anti-Gag (NIH ARP-4250), mouse anti-Gag (NIH ARP-3537 183-H12-5C), mouse anti-ALIX (1:1000, Santa Cruz Biotechnology sc-271975) and mouse anti-GFP (1:2000 Proteintech Europe 66002-1-Ig). .. The following antibodies were used for immunofluorescence experiments: rabbit anti-Gag (NIH ARP-4250), mouse anti-Env (clone 110H – Institut Pasteur), rabbit anti-CHMP4B (13683-1-AP – Proteintech). .. The following secondary antibodies were used: Dylight Alexa 488- and Cy3- and Cy5-conjugated secondary antibodies (Jackson Laboratories) were diluted 1:500.

    Article Title: Coordination of autophagosome closure and release by the Alzheimer's disease-associated protein BIN1.
    Article Snippet: Cells were imaged in a 5% CO 2 chamber at 37 ◦ C using a Zeiss LSM 780 confocal microscope with a 63 × 1.4 NA Plan Apochromat oil-immersion lens using Zen Black software. .. The primary antibodies used for immunofluorescence in HeLa and U-2 OS cells were: mouse anti-BIN1 (Santa Cruz #sc-74487, 1:200; PFA fixation); mouse anti-CD63 (Abcam #ab8219, 1:200; MeOH fixation); rabbit anti-CHMP2A (Proteintech #10477-1-AP, 1:200; PFA fixation); rabbit anti-CHMP2B (EPR10807(B)) (Abcam #ab157208, 1:200; PFA fixation); rabbit anti-CHMP4B (Proteintech #13683-1-AP, 1:200; PFA fixation); rabbit anti-DNM2 (Abcam #ab65556, 1:200; MeOH fixation); rabbit anti-DNM2 (EPR9053(2)(ABC) Abcam #ab151555; 1:200; MeOH or PFA fixation); goat anti-FLAG (Abcam #95045, 1:400; MeOH or PFA fixation); mouse anti-FLAG (Sigma #F1804, 1:400; MeOH or PFA fixation); mouse anti-GFP (Proteintech #66002-1-Ig; 1:400; MeOH or PFA fixation); rabbit antiGFP (Abcam #ab6556; 1:400; MeOH or PFA fixation); goat anti-GFP (Abcam #5450, 1:400; MeOH or PFA fixation); mouse anti-HA (Biolegend #901516, 1:400; PFA fixation); mouse anti-LC3B (Cell Signaling Technology #83506, 1:200; PFA fixation); rabbit antiLC3B (Abcam #ab192890, 1:200; MeOH or PFA fixation); mouse anti-Myc (Proteintech #60003-2-Ig; 1:400, MeOH or PFA fixation); and rabbit anti-Myc (Proteintech #16286-1-AP; 1:400, MeOH or PFA fixation); and rabbit anti-RAB11A (Abcam #ab128913, 1:200; PFA or MeOH fixation). .. The antibodies used for immunofluorescence in BV-2 microglia and mouse primary microglia were: mouse FK2 ubiquitinylated proteins (Millipore #04–263, 1:200; PFA fixation); rabbit anti-IBA1 (E404W) (Cell Signaling Technology #17198, 1:200; PFA fixation); mouse anti-FLAG (Sigma #F1804, 1:400; PFA fixation); rabbit anti-LC3B (Abcam #ab192890, 1:200; PFA fixation); and rabbit anti-p62 (MBL #PM045, 1:200; PFA fixation).

    Western Blot:

    Article Title: Coordination of autophagosome closure and release by the Alzheimer's disease-associated protein BIN1.
    Article Snippet: .. The primary antibodies used for western blotting in HeLa, H4, U-2 OS and BV-2 cells were: rabbit anti-BIN1 (Abcam #ab182562, 1:1000); rabbit anti-CHMP2A (Proteintech #10477-1-AP, 1:1000); rabbit anti-CHMP2B (EPR10807(B)) (Abcam #ab157208, 1:1000); rabbit anti-CHMP4B (Proteintech #13683-1-AP, 1:500); rabbit anti-DNM2 (Abcam #ab65556, 1:1000); rabbit anti-DNM2 (Proteintech #14605-1-AP; 1:1000); mouse anti-FLAG (Sigma #F1804, 1:5000); mouse anti-GAPDH (Proteintech #60004-1-Ig, 1:10000); mouse anti-HaloTag (Promega #G9211; 1:1000); rabbit anti-LC3B (Abcam #ab192890, 1:1000); rabbit anti-LC3B (Proteintech #14600-1- AP; 1:1000); mouse anti-Myc (Proteintech #60003-2-Ig; 1:5000); and rabbit anti-Myc (Proteintech #16286-1-AP; 1:5000), mouse anti-p62 (BD Biosciences #610833; 1:1000). .. The secondary antibodies used for western blotting in HeLa and H4 cells were: goat anti-rabbit DyLight 680 (Thermo Fisher #SA5- 10082; 1:5000) and goat anti-mouse DyLight 800 (Thermo Fisher #SA5-10156; 1:5000).

    Article Title: A  conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation
    Article Snippet: After incubated with primary and secondary antibodies, membranes were scanned using the Odyssey CLx imaging system (LI-COR Biosciences, Lincoln, Nebraska, USA). .. The following primary antibodies were used for western blotting in this study: rabbit anti-GFP (1:3,000, TP401, Torrey Pines Biolabs, Secaucus, New Jersey, USA), mouse anti-actin (1:5,000, Proteintech, Rosemont, Illinois, USA), mouse anti-LAMP1 (1:1,000, H4A3, DHSB, University of Iowa, Iowa City, Iowa, USA), mouse anti-LAMP2 (1:1,000, H4B4, DHSB), rabbit anti-CTSD (1:1,000, Cell Signaling Technology, Danvers, Massachusetts, USA), mouse anti-PDI (1:2,000, BD Biosciences, San Jose, California, USA), rabbit anti-Golgin160 (1:1,000, Proteintech), mouse-anti-EEA (1:500, G-4, Santa Cruz Biotechnology, Dallas, Texas, USA), rabbit-GAPDH (1:2,000, Proteintech), mouse anti-ubiquitin (1:100, P4D1, Santa Cruz Biotechnology), rabbit anti-ATG5 (1:2,000, D5FF5U, Cell Signaling Technology), rabbit anti-ATG7 (1:2,000, D12B11, Cell Signaling Technology), rabbit anti-FLAG (1:2,000, Millipore-Sigma), mouse anti-TSG101 (1:200, C-2, Santa Cruz Biotechnology), mouse anti-ALIX (1:500, 1A12, Santa Cruz Biotechnology), mouse anti-Vps22 (1:500, C-11, Santa Cruz Biotechnology), rabbit anti-HRS (1:2,000, D7T5N, Cell Signaling Technology), mouse anti-HA (1:1,000, 16B12, BioLegend, San Diego, California, USA), rabbit anti-CHMP4B (1:2,000, Proteintech), and rabbit anti-LAPTM4A (1:1,000, HPA, Millipore-Sigma). ..

    Article Title: A conserved ubiquitin- and ESCRT-dependent pathway to regulate human lysosomal membrane proteins
    Article Snippet: After incubated with primary and secondary antibodies, membranes were scanned using the Odyssey ® CLx imaging system (LI-COR). .. The following primary antibodies were used for Western blotting in this study: rabbit anti-GFP (1:3000, TP401, Torrey Pines Biolabs), mouse anti-actin (1:5000, Proteintech), mouse anti-LAMP1 (1:1000, H4A3, DHSB), mouse anti-LAMP2 (1:1000, H4B4, DHSB), rabbit anti-CTSD (1:1000, Cell Signaling Technology), mouse anti-PDI (1:2000, BD Biosciences), rabbit anti-Golgin160 (1:1000, Proteintech), mouse-anti-EEA (1:500, G-4, Santa Cruz Biotechnology), rabbit-GAPDH (1:2000, Proteintech), rabbit anti-ATG5 (1:2000, D5FF5U, Cell Signaling Technology), rabbit anti-ATG7 (1:2000, D12B11, Cell Signaling Technology), rabbit anti-FLAG (1:2000, Millipore-Sigma), mouse anti-TSG101 (1:200, C-2, Santa Cruz Biotechnology), mouse anti-ALIX (1:500, 1A12, Santa Cruz Biotechnology), mouse anti-Vps22 (1:500, C-11, Santa Cruz Biotechnology), mouse anti-HRS (1:500, C-7, Santa Cruz Biotechnology), mouse anti-HA (1:1000, 16B12, BioLegend), rabbit anti-CHMP4B (1:2000, Proteintech). ..

    other:

    Article Title: Non-muscle MYH10/myosin IIB recruits ESCRT-III to participate in autophagosome closure to maintain neuronal homeostasis
    Article Snippet: Rabbit anti-CHMP4B , Proteintech , 13683-1-AP, RRID:AB_2877971.



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    Proteintech rabbit polyclonal antibody against chmp4b
    Figure 7. CHMP5 activity in mammalian cells. (A) Confocal images of immunofluorescence staining against <t>CHMP4B</t> of Hela-Kyoto cells expressing CHMP5- mNeonGreen under isosmotic or hypertonic conditions. (B and C) Confocal images of immunofluorescence staining against LAMP1 or CHMP4B of Hela-Kyoto cells expressing CHMP5-mNeonGreen with or without LLOMe treatment. Zoom-in of the deconvoluted image in C is shown in Fig. 8 A. (D) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen during osmotic shock. (E) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment. (F) Quantification of experiments described in D and E. Scale bar: 10 μm; D n = 3 ROI ≥400; E n = 3 ROI ≥2,500.
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    Image Search Results


    Figure 7. CHMP5 activity in mammalian cells. (A) Confocal images of immunofluorescence staining against CHMP4B of Hela-Kyoto cells expressing CHMP5- mNeonGreen under isosmotic or hypertonic conditions. (B and C) Confocal images of immunofluorescence staining against LAMP1 or CHMP4B of Hela-Kyoto cells expressing CHMP5-mNeonGreen with or without LLOMe treatment. Zoom-in of the deconvoluted image in C is shown in Fig. 8 A. (D) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen during osmotic shock. (E) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment. (F) Quantification of experiments described in D and E. Scale bar: 10 μm; D n = 3 ROI ≥400; E n = 3 ROI ≥2,500.

    Journal: The Journal of cell biology

    Article Title: Vps60 initiates alternative ESCRT-III filaments.

    doi: 10.1083/jcb.202206028

    Figure Lengend Snippet: Figure 7. CHMP5 activity in mammalian cells. (A) Confocal images of immunofluorescence staining against CHMP4B of Hela-Kyoto cells expressing CHMP5- mNeonGreen under isosmotic or hypertonic conditions. (B and C) Confocal images of immunofluorescence staining against LAMP1 or CHMP4B of Hela-Kyoto cells expressing CHMP5-mNeonGreen with or without LLOMe treatment. Zoom-in of the deconvoluted image in C is shown in Fig. 8 A. (D) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen during osmotic shock. (E) Live-cell imaging of Hela-Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment. (F) Quantification of experiments described in D and E. Scale bar: 10 μm; D n = 3 ROI ≥400; E n = 3 ROI ≥2,500.

    Article Snippet: Rabbit monoclonal antibody against LAMP1 (1/1,000 dilution for immunofluorescence) was from Cell Signaling (9091), and rabbit polyclonal antibody against CHMP4B (1/300) was from Proteintech (13683-1-AP).

    Techniques: Activity Assay, Immunofluorescence, Staining, Expressing, Live Cell Imaging

    Figure 8. CHMP5 and CHMP4B polymers have distinct dynamics in cells. (A) Decon- voluted confocal images of immunofluores- cence staining against LAMP1 or CHMP4B (same as Fig. 7 C, higher magnification) of Hela- Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment (scale bar: 10 μm, 2 μm inlet). (B) Live-cell imaging of Hela-Kyoto cells expressing CHMP4B-GFP and CHMP5- mScarlet-I undergoing nuclear envelop refor- mation and cytokinetic abscission (scale bar: 10 μm). Arrowheads indicate the reforming nu- clear envelope and the cytokinetic bridge. (C) Cartoon of the proposed model for function of Vps60-based ESCRT-III filaments.

    Journal: The Journal of cell biology

    Article Title: Vps60 initiates alternative ESCRT-III filaments.

    doi: 10.1083/jcb.202206028

    Figure Lengend Snippet: Figure 8. CHMP5 and CHMP4B polymers have distinct dynamics in cells. (A) Decon- voluted confocal images of immunofluores- cence staining against LAMP1 or CHMP4B (same as Fig. 7 C, higher magnification) of Hela- Kyoto cells expressing CHMP5-mNeonGreen upon LLOMe treatment (scale bar: 10 μm, 2 μm inlet). (B) Live-cell imaging of Hela-Kyoto cells expressing CHMP4B-GFP and CHMP5- mScarlet-I undergoing nuclear envelop refor- mation and cytokinetic abscission (scale bar: 10 μm). Arrowheads indicate the reforming nu- clear envelope and the cytokinetic bridge. (C) Cartoon of the proposed model for function of Vps60-based ESCRT-III filaments.

    Article Snippet: Rabbit monoclonal antibody against LAMP1 (1/1,000 dilution for immunofluorescence) was from Cell Signaling (9091), and rabbit polyclonal antibody against CHMP4B (1/300) was from Proteintech (13683-1-AP).

    Techniques: Staining, Expressing, Live Cell Imaging